OXFORD NANOPORE
TECHNOLOGIES
PRODUCTs
Your personal, portable DNA and RNA sequencer. Get full control and creativity over when, where and how often you sequence. MinION Mk1B provides the power of nanopore sequencing in an affordable and completely portable device. Weighing just 100g and running from a laptop, MinION Mk1B generates tens of gigabases of data in real time, whether in the field or the laboratory.
Self-contained, easily deployable benchtop nanopore sequencer. A flexible, stand-alone, benchtop nanopore sequencer running up to five MinION or Flongle flow cells (or combinations thereof) that can respond to the needs of multiple users on demand, across varied applications. High-performance integrated data processing alleviates the need for complex computing infrastructure.
High-throughput flexible nanopore sequencers for DNA/RNA. Offering the flexibility of 24 independently controllable high-performance flow cells and leveraging cutting-edge algorithms and GPU technology, the PromethION 24 (P24) provides single or multiple users with on-demand access to terabases of sequencing data. The PromethION 48 (P48), our most powerful sequencing device, offers twice the capacity and performance of the P24, ideal for large-scale and production sequencing projects.
High-throughput flexible nanopore sequencers for DNA/RNA. Offering the flexibility of 24 independently controllable high-performance flow cells and leveraging cutting-edge algorithms and GPU technology, the PromethION 24 (P24) provides single or multiple users with on-demand access to terabases of sequencing data. The PromethION 48 (P48), our most powerful sequencing device, offers twice the capacity and performance of the P24, ideal for large-scale and production sequencing projects.
Low-cost access to high-throughput PromethION sequencing. Offering the flexibility of two independent, high-performance PromethION flow cells, the compact PromethION 2 devices bring the benefits of high-coverage nanopore sequencing to users with lower sample processing requirements. Get fully integrated sequencing and analysis with PromethION 2 Integrated, or leverage your GridION computing infrastructure with PromethION 2 Solo.
Low-cost access to high-throughput PromethION sequencing. Offering the flexibility of two independent, high-performance PromethION flow cells, the compact PromethION 2 devices bring the benefits of high-coverage nanopore sequencing to users with lower sample processing requirements. Get fully integrated sequencing and analysis with PromethION 2 Integrated, or leverage your GridION computing infrastructure with PromethION 2 Solo.
Flongle adapter is an add-on device for MinION or GridION that allows direct sequencing of DNA or RNA in real time, using smaller, single-use flow cells. Delivers up to 2.8 Gb of data. Providing immediate access to sequence data, Flongle is designed to be the fastest, most accessible and cost-effective sequencing system for smaller or more frequently performed tests or experiments. Flongle flow cells should be used within four weeks of receipt.
MinION GridION Flow Cell R10.4.1 version
The MinION flow cell (R10.4.1) is a consumable used with MinION and GridION devices. It contains the patented sensor array, application specific integrated circuit (ASIC) and R10 nanopores. The R10 nanopore series contains a dual read head and is suitable for experiments where high consensus precision is required. Paired with Chemistry Kit 14, R10.4.1 generates data with greater than 99% modal accuracy.
This version is compatible with recently released V14 kits, including the V14 Ligation Sequencing Kit, V14 Rapid Sequencing Kit, and barcoding.
MinION or GridION Flow Cell RNA
A MinION/GridION RNA flow cell is a consumable used with MinION and GridION devices. It contains the proprietary sensor array, application-specific integrated circuit (ASIC), and nanopores. This is our RNA-specific flow cell, and together with the Direct RNA Sequencing Kit (SQK-RNA004), it generates improved sequencing accuracy and output for RNA libraries.
This version is currently only compatible with our Direct RNA Sequencing Kit (SQK-RNA004).
PromethION Flow Cell R10.4.1 (M version)
A PromethION flow cell (R10.4.1) is a consumable used with PromethION 2 Solo, P24, and P48 devices. It contains the proprietary sensor array, application specific integrated circuit (ASIC) and R10 nanopores. The R10 nanopore series contains a dual read head and is suitable for experiments where high consensus precision is required. Paired with Chemistry Kit 14, R10.4.1 generates data with greater than 99% modal accuracy.
This version is compatible with recently released V14 kits, including the V14 Ligation Sequencing Kit, V14 Rapid Sequencing Kit, and barcoding. A PromethION RNA flow cell is a consumable used with PromethION 2 Solo, P24, and P48 devices.It contains the patented sensor array, Application Specific Integrated Circuit (ASIC) and nanopores. This is our RNA-specific flow cell and together with the Direct RNA Sequencing Kit (SQK-RNA004), it results in improved sequencing accuracy and throughput for RNA libraries.
Flongle Flow Cell R10.4.1 Version
A Flongle flow cell (R10.4.1) is a consumable used with the Flongle adapter and MinION and GridION devices. Contains the patented sensor array and R10.4.1 nanopores suitable for all V14 kits using Kit 14 chemistry.
This kit contains six vials of Lambda DNA to perform a Lambda control experiment. Lambda DNA is 48 kb in length and serves as a good model system to evaluate the nanopore sequencing workflow. Control experiments should be carried out when users are getting started with an Oxford Nanopore device, or when troubleshooting library preparation.
The PCR Barcoding Expansion 1-12 is designed to enable pooling and co-operation of multiple sequencing libraries on Oxford Nanopore sequencing devices. The kit includes adapters and primers for Fw and RV PCR. There are 12 unique barcodes and enough adapters to make 6 libraries per barcode. The barcodes have been carefully designed and extensively purified to minimize the possibility of crossover.
There are currently two protocols for this kit, which are provided as examples of how Barcoding Expansion 1-12 PCR could be applied for genomic and amplicon libraries. Please note that if you are encoding cDNA or amplicons, these have to be prepared beforehand. These protocols are open to adaptation and optimization for specific experimental requirements. Please note that to use this kit, you will need to purchase additional reagents from a third party.
The PCR Barcoding Expansion 1-96 (EXP-PBC096) is designed to allow pooling and working together of multiple sequencing libraries. The kit includes RV and FW PCR adapters and primers with the same barcode on both primers. The kit contains 96 unique barcodes. There are 96 unique barcodes and enough of the adapters needed to make 10 libraries per barcode. The barcodes have been carefully designed and extensively purified to minimize the possibility of interference.
There are currently two protocols for this kit that are provided as examples of how Barcoding Expansion 1-96 PCR could be applied for genomic and amplicon libraries. Please note that if you are encoding cDNA or amplicons, these have to be prepared beforehand. These protocols are open to adaptation and optimization for specific experimental requirements. Please note that to use this kit, you will need to purchase additional reagents from a third party.
This kit is recommended for users who:
-Wish to wash flow cells and rerun with fresh samples.
-Want to process samples on demand, rather than processing them in batches.
-They have accumulated a large number of “unavailable” pores during a previous run and want to revert these pores to the “single pore” state.
This kit is recommended for users who:
-Wish to wash flow cells and rerun with fresh samples.
-Want to process samples on demand, rather than processing them in batches.
-They have accumulated a large number of “unavailable” pores during a previous run and want to revert these pores to the “single pore” state.
The Native Barcoding Auxiliary V14 Kit (EXP-NBA114) contains additional library preparation reagents for the Native Barcoding Kit 24 and 96 V14 (SQK-NBD114.24 and SQK-NBD114.96), and the Multiplex Ligation Sequencing Kit XL V14 ( SQK-MLK114.96-XL), for another 12 reactions. This way, users can use unused barcodes in their native barcode kits.
To use this kit, be sure to purchase the V14 Sequencing Auxiliary Vials (EXP-AUX003) to obtain the flow cell priming reagents.
This kit contains PCR primers and adapters and is compatible for use with ligation sequencing kits (SQK-LSK110 and SQK-LSK109).
This kit is recommended for users who:
-Want to split a library into multiple flow cells.
-Need to add a new library to a washed flow cell.
-Want to populate a flow cell with an additional library.
This kit is recommended for users who:
-They want to select by size to progressively eliminate short fragments (<25 kb) from their sample.
-Want to enrich their libraries for long fragments without using external third-party reagents.
The Short Fragment Eliminator (EXP-SFE001) is a new expansion pack recommended for users who wish to size select high molecular weight (HMW) gDNA to eliminate short fragments (<25 kb) by size-selective precipitation. The kit contains the Short Fragment Eliminator (SFE) buffer that progressively removes fragments up to 25 kb, almost completely removing fragments smaller than 10 kb in a fast and easy workflow.
The SFB Expansion kit contains additional Short Fragment Buffer (SFB) for use with sequencing experiments where short fragments are required or for use with the “PCR tiling of COVID-19 virus” protocol.
The LFB expansion kit contains additional long fragment buffer (LFB) for use with sequencing experiments where shorter fragments (<3 kb) must be removed.
This kit is recommended for users who:
-Want to sequence the complete genome of SARS-CoV-2-Want to multiplex samples to reduce the price per sample
The Midnight RT PCR Expansion (EXP-MRT001) is designed to be used with the Rapid Barcoding Kit 96 (SQK-RBK110.96) or the Rapid Barcoding Kit 96 V14 (SQK-RBK114.96) and provides all reagents necessary for the Complete workflow for sequencing SARS-CoV-2 RNA samples.
The RNA Control Expansion contains RNA control samples for control testing of cDNA sequencing kits.
The Rapid Adapter Auxiliary V14 expansion (EXP-RAA114) contains additional rapid adapter and adapter buffers for six additional reactions. This allows users to use any unused barcodes on their Rapid Barcoding Kits V14.
To use this kit, be sure to purchase the V14 Sequencing Auxiliary Vials (EXP-AUX003) to obtain the flow cell priming reagents.
The Rapid Adapter Auxiliary V14 (EXP-RAA114) can only be used with chemistry 14 kis. This kit is NOT compatible with other kits based on previous chemistries.
This extension is recommended for users who:
-Wish to perform additional flow cell loading from existing ultralong library preparations.
-Ultra-Long Auxiliary Vials (EXP-ULA001) contain enough reagents for 12 additional reactions.
The EEB expansion contains additional reagents for use with the V14 Ultra Long DNA Sequencing Kit (SQK-ULK114).
The Flow Cell Priming Kit contains additional flow cell priming reagents for six reactions using the V14 kits.
The kit can be used to prime MinION and GridION or PromethION flow cells for sequencing or to load additional libraries into a flow cell during a sequencing experiment.
Note: Only compatible with V14 chemistry.
The Flow Cell Priming Kit contains additional flow cell priming reagents for six reactions using the V14 kits.
The kit can be used to prime MinION and GridION or PromethION flow cells for sequencing or to load additional libraries into a flow cell during a sequencing experiment.
Note: Only compatible with V14 chemistry.
This kit is recommended for users who:
-Desire to obtain raw modal reading accuracy of Q20+ (99%) and higher
-Want to obtain duplex data
-Want to optimize your sequencing experiment for accuracy and performance
-They need to control the length of the readings
-They want to use previous processes such as size selection or complete genome amplification.
Please note that to use this kit, you will need to purchase additional reagents from a third party.
Ligation Sequencing Kit XL V14
This kit is recommended for users who:
-Desire to obtain raw modal reading accuracy of Q20+ (99%) and higher
-Want to obtain duplex data
-Want to optimize your sequencing experiment for accuracy and performance
-They need to control the length of the readings
-They want to use previous processes such as size selection or complete genome amplification.
Please note that to use this kit, you will need to purchase additional reagents from a third party.
This kit is recommended for users who:
-Want to multiplex up to 24 samples to reduce the price per sample
-Want to achieve raw modal reading accuracy of Q20+ (99%) and higher
-Want to obtain duplex data
-Want to optimize your sequencing experiment for accuracy and performance
-They need a multiplexing method without PCR to preserve additional information such as base modifications
-They need to control the length of the reading
-They want to use previous processes such as size selection or complete genome amplification
Please note that to use this kit, you will need to purchase additional third-party reagents.
This kit is recommended for users who:
-Want to multiplex up to 24 samples to reduce the price per sample
-Want to achieve raw modal reading accuracy of Q20+ (99%) and higher
-Want to obtain duplex data
-Want to optimize your sequencing experiment for accuracy and performance
-They need a multiplexing method without PCR to preserve additional information such as base modifications
-They need to control the length of the reading
-They want to use previous processes such as size selection or complete genome amplification
Please note that to use this kit, you will need to purchase additional third-party reagents.
Multiplex Ligation Sequencing Kit XL V14
This kit is recommended for users who:
-Want to optimize the performance of your sequencing experiment
-Desire low multiplex samples for whole genome sequencing (WGS)
-Need a PCR-free multiplexing method to preserve additional information, such as base modifications
-Need to control the length of the readings
-You want to use upstream processes, such as size selection or whole genome amplification
-You would like to process multiple samples simultaneously, either with a multichannel pipettor
Please note that to use this kit, you will need to purchase additional third-party reagents.
This kit is recommended for users who:
-Require little preparation time
-Have limited access to laboratory equipment
Please note that to use this kit, you will need to purchase additional reagents from third parties.
Rapid PCR Barcoding Kit 24 V14
This kit is recommended for users who:
-Want to multiplex up to 24 samples to reduce the price per sample
-They have a low initial amount of gDNA
-Require a simple library preparation procedure
Please note that to use this kit, you will need to purchase additional reagents from a third party.
This kit is recommended for users who:
-Wish to perform 16S sequencing
-are interested in the identification of bacteria at the genus level
Please note that to use this kit, you will need to purchase additional reagents from third parties.
This kit is recommended for users who:
-They have a limited amount of input material
-Want to multiplex samples to reduce the price per sample
-Want to optimize the performance of your sequencing experiment
-Want to identify and quantify full-length transcripts
-Are interested in differential gene expression
-They wish to characterize and quantify isoforms, splice variants and fusion transcripts
Please note that to use this kit, you will need to purchase additional third-party reagents.
This kit is recommended for users who:
-They have a limited amount of input material
-Want to optimize the result of your sequencing experiment
-Want to identify and quantify full-length transcripts
-Are interested in differential gene expression
-Wish to characterize and quantify isoforms, splice variants and fusion transcripts
Please note that to use this kit you will need to purchase additional third-party reagents.
This kit is highly recommended for users who:
-Want to eliminate PCR bias
-Explore the attributes of native RNA, such as modified bases.
Please note that to use this kit you will need to purchase additional third-party reagents.
Rapid barcoding sequencing kit 24 V14
This kit is recommended for users who:
-Want to multiplex up to 24 samples to reduce the price per sample
-Require a short preparation time
-Need a PCR-free multiplexing method to preserve additional information, such as base modifications
-Have limited access to laboratory equipment
Please note that to use this kit you will need to purchase additional third-party reagents.
Rapid barcoding sequencing kit 96 V14
This kit is recommended for users who:
-Want to multiplex up to 96 samples to reduce the price per sample
-Require a short preparation time
-Need a PCR-free multiplexing method to preserve additional information, such as base modifications
-Have limited access to laboratory equipment
Please note that to use this kit you will need to purchase additional third-party reagents.
Ultra-Long DNA Sequencing Kit V14
This kit is recommended for users who:
-Work with ultra-high molecular weight DNA and want read lengths with an N50 >50 kb.
-Require an end-to-end workflow to sequence ultra-high molecular weight DNA.
Please note that to use this kit you will need to purchase additional third-party reagents.